The IVF Process — Step 4: Sperm Collection & Processing

A successful In Vitro Fertilization outcome relies heavily on selecting healthy paternal genetic material. At Akanksha IVF Centre, recognized as the best IVF centre in Delhi for male fertility solutions and andrology tracking, the collection and preparation of the semen sample occur under highly standardized laboratory settings directly parallel to the egg retrieval window.

Semen Collection Protocols

On the morning of the egg retrieval, the male partner or selected donor provides a fresh sample in our secure, private andrology suites. To ensure optimal sample parameters, we advise a strict window of 2 to 5 days of sexual abstinence prior to this collection date.

• Advanced Extraction Alternatives: For couples navigating absolute male-factor obstacles, our clinic provides specialized surgical sperm extraction solutions. These micro-surgical options include Testicular Sperm Extraction (TESE) and Percutaneous Epididymal Sperm Aspiration (PESA), conducted safely by our staff.
• Frozen and Donor Alignments: If you are utilizing cryopreserved (frozen) partner sperm or a verified donor sample, our embryologists carefully pull the vials from our secure storage tanks and execute precise thawing protocols at this exact clinical juncture.

Sperm Washing and Isolation Techniques

Raw semen contains seminal plasma, cellular debris, and immobile cells that cannot be introduced directly to an egg in a laboratory dish. Our internal embryology department applies specialized purification steps to isolate the healthiest cells:

We process the sample using a high-performance Density Gradient Centrifugation technique. This separates out dead cells and seminal fluids, shifting the highest-quality cells to the base of the tube. This method concentrates a highly active pool of sperm that display healthy shape and rapid progressive movement, setting up the cycle for successful fertilization.